Main Logo
Logo

Society for Pediatric Radiology – Poster Archive

  329
  0
  0
 
 


Final ID: Paper #: 154

Non-invasive Imaging of Ferumoxytol Labeled Chimeric Antigen Receptor (CAR) T-cells Trafficking to Osteosarcoma

Purpose or Case Report: Metastasized osteosarcoma has a poor prognosis with a two-year event free survival rate of 15-20%, highlighting the need for the advancement of efficacious therapeutics. Chimeric antigen receptor (CAR) T-cell therapy is emerging as a potent strategy for eliminating tumors by harnessing the immune system. Despite this, trials encounter challenges due to varied responses and failing therapy in patients. Development of translatable cell imaging methods would provide invaluable information regarding the biological fate of T-cells. This work aims to evaluate a new approach for CAR T-cell labeling with ferumoxytol, which enables non-invasive cell tracking with magnetic resonance imaging (MRI) and a new imaging technique, magnetic particle imaging (MPI).
Methods & Materials: In vitro characterization: We isolated human T-cells from donors, expressed the CAR gene and stimulated for expansion. A new microfluidics device was used to label T-cells with ferumoxytol nanoparticles (Feraheme) through cell volume exchange for connective transfer. The iron content of labeled T-cells and unlabeled controls was analyzed by prussian blue histology and inductively coupled plasma optical emission spectrometry. To determine the sensitivity, varying numbers (103-106) of labeled T-cells and controls were imaged using MRI or MPI. In vivo imaging: Labeled or unlabeled T-cells were intravenously administered in osteosarcoma bearing NOD SCID mice and animals were imaged using MRI and MPI. The MPI signal intensity and T2 relaxation times of different groups were compared using an analysis of variance (ANOVA) and a linear regression analysis.
Results: In vitro characterization: Retention of ferumoxytol was significantly (p<0.01) greater in labeled T-cells (0.39 ± 0.003 pg/cell) than controls (0.06 ± 0.006 pg/cell). 104 ferumoxytol labeled T-cells were visually detected using MPI and MRI. T2 relaxation times of labeled T-cells were significantly shorter compared to unlabeled for cell numbers ≥104. The ferumoxytol MPI signal showed a linear correlation with the T-cell number. In vivo imaging: MPI demonstrated homing of T-cells to osteosarcoma in animals administered with labeled T-cells, whilst T-cells were not visualized in animals infused with unlabeled cells.
Conclusions: This study demonstrates the first successful labeling of CAR T-cells with ferumoxytol. The labeled cells could be detected in vitro and in vivo, using MRI and MPI, thereby paving the way for in vivo monitoring of CAR T cell trafficking to osteosarcomas.
  • Kiru, Louise  ( Stanford University School of Medicine , Stanford , California , United States )
  • Wu, Wei  ( Stanford University School of Medicine , Stanford , California , United States )
  • Nejadnik, Hossein  ( University of Arizona , Tucson , Arizona , United States )
  • Liu, Anna  ( Georgia Institute of Technology , Atlanta , Georgia , United States )
  • Majzner, Robbie  ( Stanford University School of Medicine , Stanford , California , United States )
  • Sulchek, Todd  ( Georgia Institute of Technology , Atlanta , Georgia , United States )
  • Daldrup-link, Heike  ( Stanford University School of Medicine , Stanford , California , United States )
Session Info:

Scientific Session VI-A: Nuclear Medicine/Oncology

Nuclear Imaging/Oncology

SPR Scientific Papers

More abstracts on this topic:
Noninvasive Tracking of Disease Progression in TH-MYCN Mouse Models of Neuroblastoma Using Multi-modal Imaging

Badachhape Andrew, Tao Ling, Joshi Sanshuv, Devkota Laxman, Stupin Igor, Sarkar Poonam, Annapragada Ananth, Barbieri Eveline, Ghaghada Ketan

The Correlation Between the First Order CT Features in Pre-treatment CT Texture and MYCN Amplification Status in Neuroblastoma

Marie Eman, Oudjhane Kamaldine

More abstracts from these authors:
PET/MRI of Children with Cancer: Your Key To Success

Jayapal Praveen, Baratto Lucia, Rashidi Ali, Daldrup-link Heike

Applications of Artificial Intelligence in Staging and Re-Staging of Pediatric Cancers

Sarrami Amir Hossein, Wang Hongzhi, Baratto Lucia, Syeda-mahmood Tanveer, Daldrup-link Heike

Due to circumstances surrounding the coronavirus pandemic, this final ePoster exhibit was not submitted.
You have to be authorized to contact abstract author. Please, Login or Signup.

Please note that this is a separate login, not connected with your credentials used for the SPR main website.

Not Available

Comments

We encourage you to join the discussion by posting your comments and questions below.

Presenters will be notified of your post so that they can respond as appropriate.

This discussion platform is provided to foster engagement, and stimulate conversation and knowledge sharing.

Please click here to review the full terms and conditions for engaging in the discussion, including refraining from product promotion and non-constructive feedback.

 

You have to be authorized to post a comment. Please, Login or Signup.

Please note that this is a separate login, not connected with your credentials used for the SPR main website.


   Rate this abstract  (Maximum characters: 500)